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pegfp n1 tfeb  (Addgene inc)


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    Structured Review

    Addgene inc pegfp n1 tfeb
    Pegfp N1 Tfeb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+tfe3/pEGFP-N1-TFE3+(Plasmid+%2338120)/pmc12618667-480-117-118
    Average 93 stars, based on 24 article reviews
    pegfp n1 tfeb - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Construct:

    Article Title: Lysine-arginine imbalance overcomes therapeutic tolerance governed by the transcription factor E3-lysosome axis in glioblastoma.
    Article Snippet: A full-length human MGMT expression construct was generated by subcloning the cDNA from MGMT-expressing GBM cells into the pLJM1 lentiviral vector in which a CMV promoter was exchanged for an EF1 promoter from lentiGuide-Puro (lentiGuide-Puro was a gift from Feng Zhang [Addgene plasmid No. 52963; http://n2t.net/addgene:52963; RRID:Addgene_52963]62). .. The pLJM1-TFE3-EGFP construct was generated by subcloning the TFE3EGFP from pEGFP-N1-TFE3 (pEGFP-N1-TFE3 was a gift from Shawn Ferguson [Addgene plasmid No. 38120; http://n2t.net/addgene: 38120; RRID:Addgene_38120]63) into the pLJM1 lentiviral vector. .. Single guide (sg) RNA constructs against human sequences were generated by ligation of annealing oligonucleotides into the BsmBI site of the plentiCRISPR-v2 vector (lentiCRISPR v2 was a gift from Feng Zhang [Addgene plasmid No. 52961]62).

    Article Title: Lysine-arginine imbalance overcomes therapeutic tolerance governed by the transcription factor E3-lysosome axis in glioblastoma
    Article Snippet: A full-length human MGMT expression construct was generated by subcloning the cDNA from MGMT -expressing GBM cells into the pLJM1 lentiviral vector in which a CMV promoter was exchanged for an EF1 promoter from lentiGuide-Puro (lentiGuide-Puro was a gift from Feng Zhang [Addgene plasmid No. 52963; http://n2t.net/addgene:52963 ; RRID:Addgene_52963] ). .. The pLJM1-TFE3-EGFP construct was generated by subcloning the TFE3 -EGFP from pEGFP-N1-TFE3 (pEGFP-N1-TFE3 was a gift from Shawn Ferguson [Addgene plasmid No. 38120; http://n2t.net/addgene:38120 ; RRID:Addgene_38120] ) into the pLJM1 lentiviral vector. .. Single guide (sg) RNA constructs against human sequences were generated by ligation of annealing oligonucleotides into the BsmBI site of the plentiCRISPR-v2 vector (lentiCRISPR v2 was a gift from Feng Zhang [Addgene plasmid No. 52961] ).

    Generated:

    Article Title: Lysine-arginine imbalance overcomes therapeutic tolerance governed by the transcription factor E3-lysosome axis in glioblastoma.
    Article Snippet: A full-length human MGMT expression construct was generated by subcloning the cDNA from MGMT-expressing GBM cells into the pLJM1 lentiviral vector in which a CMV promoter was exchanged for an EF1 promoter from lentiGuide-Puro (lentiGuide-Puro was a gift from Feng Zhang [Addgene plasmid No. 52963; http://n2t.net/addgene:52963; RRID:Addgene_52963]62). .. The pLJM1-TFE3-EGFP construct was generated by subcloning the TFE3EGFP from pEGFP-N1-TFE3 (pEGFP-N1-TFE3 was a gift from Shawn Ferguson [Addgene plasmid No. 38120; http://n2t.net/addgene: 38120; RRID:Addgene_38120]63) into the pLJM1 lentiviral vector. .. Single guide (sg) RNA constructs against human sequences were generated by ligation of annealing oligonucleotides into the BsmBI site of the plentiCRISPR-v2 vector (lentiCRISPR v2 was a gift from Feng Zhang [Addgene plasmid No. 52961]62).

    Article Title: Lysine-arginine imbalance overcomes therapeutic tolerance governed by the transcription factor E3-lysosome axis in glioblastoma
    Article Snippet: A full-length human MGMT expression construct was generated by subcloning the cDNA from MGMT -expressing GBM cells into the pLJM1 lentiviral vector in which a CMV promoter was exchanged for an EF1 promoter from lentiGuide-Puro (lentiGuide-Puro was a gift from Feng Zhang [Addgene plasmid No. 52963; http://n2t.net/addgene:52963 ; RRID:Addgene_52963] ). .. The pLJM1-TFE3-EGFP construct was generated by subcloning the TFE3 -EGFP from pEGFP-N1-TFE3 (pEGFP-N1-TFE3 was a gift from Shawn Ferguson [Addgene plasmid No. 38120; http://n2t.net/addgene:38120 ; RRID:Addgene_38120] ) into the pLJM1 lentiviral vector. .. Single guide (sg) RNA constructs against human sequences were generated by ligation of annealing oligonucleotides into the BsmBI site of the plentiCRISPR-v2 vector (lentiCRISPR v2 was a gift from Feng Zhang [Addgene plasmid No. 52961] ).

    Subcloning:

    Article Title: Lysine-arginine imbalance overcomes therapeutic tolerance governed by the transcription factor E3-lysosome axis in glioblastoma.
    Article Snippet: A full-length human MGMT expression construct was generated by subcloning the cDNA from MGMT-expressing GBM cells into the pLJM1 lentiviral vector in which a CMV promoter was exchanged for an EF1 promoter from lentiGuide-Puro (lentiGuide-Puro was a gift from Feng Zhang [Addgene plasmid No. 52963; http://n2t.net/addgene:52963; RRID:Addgene_52963]62). .. The pLJM1-TFE3-EGFP construct was generated by subcloning the TFE3EGFP from pEGFP-N1-TFE3 (pEGFP-N1-TFE3 was a gift from Shawn Ferguson [Addgene plasmid No. 38120; http://n2t.net/addgene: 38120; RRID:Addgene_38120]63) into the pLJM1 lentiviral vector. .. Single guide (sg) RNA constructs against human sequences were generated by ligation of annealing oligonucleotides into the BsmBI site of the plentiCRISPR-v2 vector (lentiCRISPR v2 was a gift from Feng Zhang [Addgene plasmid No. 52961]62).

    Article Title: Lysine-arginine imbalance overcomes therapeutic tolerance governed by the transcription factor E3-lysosome axis in glioblastoma
    Article Snippet: A full-length human MGMT expression construct was generated by subcloning the cDNA from MGMT -expressing GBM cells into the pLJM1 lentiviral vector in which a CMV promoter was exchanged for an EF1 promoter from lentiGuide-Puro (lentiGuide-Puro was a gift from Feng Zhang [Addgene plasmid No. 52963; http://n2t.net/addgene:52963 ; RRID:Addgene_52963] ). .. The pLJM1-TFE3-EGFP construct was generated by subcloning the TFE3 -EGFP from pEGFP-N1-TFE3 (pEGFP-N1-TFE3 was a gift from Shawn Ferguson [Addgene plasmid No. 38120; http://n2t.net/addgene:38120 ; RRID:Addgene_38120] ) into the pLJM1 lentiviral vector. .. Single guide (sg) RNA constructs against human sequences were generated by ligation of annealing oligonucleotides into the BsmBI site of the plentiCRISPR-v2 vector (lentiCRISPR v2 was a gift from Feng Zhang [Addgene plasmid No. 52961] ).

    Plasmid Preparation:

    Article Title: Lysine-arginine imbalance overcomes therapeutic tolerance governed by the transcription factor E3-lysosome axis in glioblastoma.
    Article Snippet: A full-length human MGMT expression construct was generated by subcloning the cDNA from MGMT-expressing GBM cells into the pLJM1 lentiviral vector in which a CMV promoter was exchanged for an EF1 promoter from lentiGuide-Puro (lentiGuide-Puro was a gift from Feng Zhang [Addgene plasmid No. 52963; http://n2t.net/addgene:52963; RRID:Addgene_52963]62). .. The pLJM1-TFE3-EGFP construct was generated by subcloning the TFE3EGFP from pEGFP-N1-TFE3 (pEGFP-N1-TFE3 was a gift from Shawn Ferguson [Addgene plasmid No. 38120; http://n2t.net/addgene: 38120; RRID:Addgene_38120]63) into the pLJM1 lentiviral vector. .. Single guide (sg) RNA constructs against human sequences were generated by ligation of annealing oligonucleotides into the BsmBI site of the plentiCRISPR-v2 vector (lentiCRISPR v2 was a gift from Feng Zhang [Addgene plasmid No. 52961]62).

    Article Title: Lysine-arginine imbalance overcomes therapeutic tolerance governed by the transcription factor E3-lysosome axis in glioblastoma
    Article Snippet: A full-length human MGMT expression construct was generated by subcloning the cDNA from MGMT -expressing GBM cells into the pLJM1 lentiviral vector in which a CMV promoter was exchanged for an EF1 promoter from lentiGuide-Puro (lentiGuide-Puro was a gift from Feng Zhang [Addgene plasmid No. 52963; http://n2t.net/addgene:52963 ; RRID:Addgene_52963] ). .. The pLJM1-TFE3-EGFP construct was generated by subcloning the TFE3 -EGFP from pEGFP-N1-TFE3 (pEGFP-N1-TFE3 was a gift from Shawn Ferguson [Addgene plasmid No. 38120; http://n2t.net/addgene:38120 ; RRID:Addgene_38120] ) into the pLJM1 lentiviral vector. .. Single guide (sg) RNA constructs against human sequences were generated by ligation of annealing oligonucleotides into the BsmBI site of the plentiCRISPR-v2 vector (lentiCRISPR v2 was a gift from Feng Zhang [Addgene plasmid No. 52961] ).

    Article Title: Regulation of lipoprotein processing by GPNMB in foamy macrophages: potential therapeutic targets for atherosclerosis
    Article Snippet: To generate a pEGFP-GPNMB fusion plasmid and a pEGFP-TFEC fusion plasmid, the ORF of mouse Gpnmb and human Tfec was subcloned into pEGFP-N3 (purchased from TSINGKE, China). mEmerald–RAB5A and mCherry–KDEL plasmids were gifts from Prof. Li Dong (National Laboratory of Biomacromolecules). σ2-mCherry was a gift from Stephen Royle (Addgene plasmid #186579; http://n2t.net/addgene:186579 ; RRID:Addgene_186579) . .. BFP-Sec61 beta was a gift from Gia Voeltz (Addgene plasmid #49154; http://n2t.net/addgene:49154 ; RRID:Addgene_49154) . mApple-Moesin-N-14 was a gift from Michael Davidson (Addgene plasmid #54928; http://n2t.net/addgene:54928 ; RRID:Addgene_54928). mPlum-Rab11a-7 was a gift from Michael Davidson (Addgene plasmid #55998; http://n2t.net/addgene:55998 ; RRID:Addgene_55998). mEos2-NEEP21-N-18 was a gift from Michael Davidson (Addgene plasmid #57407; http://n2t.net/addgene:57407 ; RRID:Addgene_57407). mCherry-MannII-N-10 was a gift from Michael Davidson (Addgene plasmid #55074; http://n2t.net/addgene:55074 ; RRID:Addgene_55074). ptdTomato-N1-FLCN was a gift from Shawn Ferguson (Addgene plasmid #49174; http://n2t.net/addgene:49174 ; RRID:Addgene_49174) . mCh-KIF5B*-strep was a gift from Juan Bonifacino (Addgene plasmid #120164; http://n2t.net/addgene:120164 ; RRID:Addgene_120164) . pCAG R2pH-LAMP1-3xFLAG was a gift from Massimiliano Stagi (Addgene plasmid #157940; http://n2t.net/addgene:157940 ; RRID:Addgene_157940) . pEGFP-N1-MITF-M (Addgene plasmid #38131; http://n2t.net/addgene:38131 ; RRID:Addgene_38131), pEGFP-N1-TFEB (Addgene plasmid #38119; http://n2t.net/addgene:38119 ; RRID:Addgene_38119), and pEGFP-N1-TFE3 (Addgene plasmid #38120; http://n2t.net/addgene:38120 ; RRID:Addgene_38120) were gifts from Shawn Ferguson . .. Monoclonal antibodies against mouse GPNMB (ab188222), GFP (ab183734), TFEC (ab185226), SEC22B (ab181076), ATP1A1 + ATP1A2 + ATP1A3 + ATP1A4 (ab300507), Rab5 (ab218624), LAMP1 (ab289548), and F4/80 (ab300421, Abcam) were purchased from Abcam.

    Article Title: Impairment of lysosomal quality control in Huntington disease
    Article Snippet: .. FLAG-TFEB and EGFP-TFEB plasmids were kindly provided by Prof. Andrea Ballabio (Telethon Institute of Genetics and Medicine (TIGEM), Dulbecco Telethon Institute, Federico II University, Naples). pEGFP-N1-TFE3 was a gift from Dr. Shawn Ferguson (Addgene plasmid #38120; http://n2t.net/addgene:38120 ; RRID:Addgene_38120). ..

    Article Title: Blockage of Akt activation suppresses cadmium-induced renal tubular cellular damages through aggrephagy in HK-2 cells
    Article Snippet: GAPDH (GT239) and MITF antibodies were obtained from Genetex Inc. (Irvine, CA, USA). .. The siRNAs targeted against the human TFEB (siRNA-1: HSS111868 Stealth siRNA, siRNA-2: HSS111870 Stealth siRNA), TFE3 (siRNA-1: s14031 SilencerTM Select siRNA, siRNA-2: s14031 SilencerTM Select siRNA), MITF (siRNA: s8792 SilencerTM Select siRNA), SilencerTM select Negative Control No.1 siRNA, and Stealth RNAiTM siRNA Negative Control Med GC were purchased from Thermo Fisher Scientific. pcDNA3.1-TFEB-WT-MYC (Addgene plasmid 99955) was a gift from James Brugarolas. pEGFP-N1-TFE3 (Addgene plasmid 38120) and pEGFP-N1-MITF-D (Addgene plasmid 38133) were gifts from Shawn Ferguson. .. HK-2 cells were obtained from the American Type Culture Collection (Manassas, VA, USA) and grown in Dulbecco’s modified Eagle’s medium/Nutrient Mixture F-12 supplemented with 10% heat-inactivated fetal bovine serum, 100 U/ml penicillin, and 100 μg/ml streptomycin (GIBCO, Invitrogen Corp., Carlsbad, CA, USA) in a humidified atmosphere of 5% CO 2 and 95% air at 37 °C.

    Transfection:

    Article Title: TFE3 fusion oncoprotein condensates drive transcriptional reprogramming and cancer progression in translocation renal cell carcinoma.
    Article Snippet: The following RNA FISH Probes were used: Human NEAT1 with Quasar 570 Dye (ISMF-2037-1, Biosearch Technologies), Human ACTB intron for iceFISH with Quasar 570 Dye (ISMF-2002-5m Biosearch Technologies), Custom Human GPNMB intron with Quasar 670 Dye (custom against intron 1, Biosearch Technologies). .. Transfection of U-2 OS cells Unless otherwise indicated, full-length and domain deletion mutants of NONO::TFE3 and SFPQ::TFE3 were from Genscript, on a pcDNA3.1(+)-C-eGFP vector. pEGFP-N1-TFE3 was a gift from Shawn Ferguson (Addgene #38120). .. Unless otherwise indicated, we used Q5 to perform PCR andHiFi DNA Assembly (E2621X, NEB) to assemble the other custom constructs, according to themanufacturer’s instructions.

    Negative Control:

    Article Title: Blockage of Akt activation suppresses cadmium-induced renal tubular cellular damages through aggrephagy in HK-2 cells
    Article Snippet: GAPDH (GT239) and MITF antibodies were obtained from Genetex Inc. (Irvine, CA, USA). .. The siRNAs targeted against the human TFEB (siRNA-1: HSS111868 Stealth siRNA, siRNA-2: HSS111870 Stealth siRNA), TFE3 (siRNA-1: s14031 SilencerTM Select siRNA, siRNA-2: s14031 SilencerTM Select siRNA), MITF (siRNA: s8792 SilencerTM Select siRNA), SilencerTM select Negative Control No.1 siRNA, and Stealth RNAiTM siRNA Negative Control Med GC were purchased from Thermo Fisher Scientific. pcDNA3.1-TFEB-WT-MYC (Addgene plasmid 99955) was a gift from James Brugarolas. pEGFP-N1-TFE3 (Addgene plasmid 38120) and pEGFP-N1-MITF-D (Addgene plasmid 38133) were gifts from Shawn Ferguson. .. HK-2 cells were obtained from the American Type Culture Collection (Manassas, VA, USA) and grown in Dulbecco’s modified Eagle’s medium/Nutrient Mixture F-12 supplemented with 10% heat-inactivated fetal bovine serum, 100 U/ml penicillin, and 100 μg/ml streptomycin (GIBCO, Invitrogen Corp., Carlsbad, CA, USA) in a humidified atmosphere of 5% CO 2 and 95% air at 37 °C.



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